Immunofluorescence:Article Title: Myogenic Differentiation (MyoD) Gene Expression in Cornea and Role in Corneal Myofibroblast Dedifferentiation
Article Snippet: .. Immunofluorescence was used to detect the level, localization, and distribution of MyoD and αSMA proteins in non-fibrotic and fibrotic rabbit and human corneas, as well as in vitro CSFs and CMFs, using antibodies specific to MyoD (sc-377460; Santa Cruz Biotechnology, Dallas, TX, USA) and αSMA (M0851; Dako, Glostrup, Denmark). .. For dedifferentiation studies, immunostaining was performed with the Ki67 (9129T; Cell Signaling Technology, Danvers, MA, USA), FSP1 (ab-41532; Abcam, Cambridge, UK), and intermediate filaments, such as Invitrogen Vimentin Polyclonal Antibody (PA1-16759; Thermo Fisher Scientific) and Invitrogen Desmin Polyclonal Antibody (PA5-19063; Thermo Fisher Scientific), in CMFs with or without MyoD sh RNA or scrambled sh RNA.
Article Title: Myogenic Differentiation (MyoD) Gene Expression in Cornea and Role in Corneal Myofibroblast Dedifferentiation.
Article Snippet: .. Immunofluorescence Immunofluorescence was used to detect the level, localization, and distribution of MyoD and αSMA proteins in non-fibrotic and fibrotic rabbit and human corneas, as well as in vitro CSFs and CMFs, using antibodies specific to MyoD (sc-377460; Santa Cruz Biotechnology, Dallas, TX, USA) and αSMA (M0851; Dako, Glostrup, Denmark). .. For dedifferentiation studies, immunostaining was performed with the Ki67 (9129T; Cell Signaling Technology, Danvers, MA, USA), FSP1 (ab-41532; Abcam, Cambridge, UK), and intermediate filaments, such as Invitrogen Vimentin Polyclonal Antibody (PA1-16759; Thermo Fisher Scientific) and Invitrogen Desmin Polyclonal Antibody (PA5-19063; Thermo Fisher Scientific), in CMFs with or without MyoD shRNA or scrambled shRNA.
In Vitro:Article Title: Myogenic Differentiation (MyoD) Gene Expression in Cornea and Role in Corneal Myofibroblast Dedifferentiation
Article Snippet: .. Immunofluorescence was used to detect the level, localization, and distribution of MyoD and αSMA proteins in non-fibrotic and fibrotic rabbit and human corneas, as well as in vitro CSFs and CMFs, using antibodies specific to MyoD (sc-377460; Santa Cruz Biotechnology, Dallas, TX, USA) and αSMA (M0851; Dako, Glostrup, Denmark). .. For dedifferentiation studies, immunostaining was performed with the Ki67 (9129T; Cell Signaling Technology, Danvers, MA, USA), FSP1 (ab-41532; Abcam, Cambridge, UK), and intermediate filaments, such as Invitrogen Vimentin Polyclonal Antibody (PA1-16759; Thermo Fisher Scientific) and Invitrogen Desmin Polyclonal Antibody (PA5-19063; Thermo Fisher Scientific), in CMFs with or without MyoD sh RNA or scrambled sh RNA.
Article Title: Myogenic Differentiation (MyoD) Gene Expression in Cornea and Role in Corneal Myofibroblast Dedifferentiation.
Article Snippet: .. Immunofluorescence Immunofluorescence was used to detect the level, localization, and distribution of MyoD and αSMA proteins in non-fibrotic and fibrotic rabbit and human corneas, as well as in vitro CSFs and CMFs, using antibodies specific to MyoD (sc-377460; Santa Cruz Biotechnology, Dallas, TX, USA) and αSMA (M0851; Dako, Glostrup, Denmark). .. For dedifferentiation studies, immunostaining was performed with the Ki67 (9129T; Cell Signaling Technology, Danvers, MA, USA), FSP1 (ab-41532; Abcam, Cambridge, UK), and intermediate filaments, such as Invitrogen Vimentin Polyclonal Antibody (PA1-16759; Thermo Fisher Scientific) and Invitrogen Desmin Polyclonal Antibody (PA5-19063; Thermo Fisher Scientific), in CMFs with or without MyoD shRNA or scrambled shRNA.
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